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An integrated approach for an interoperable industrial networking architecture consisting of heterogeneous fieldbuses??Original Research ArticleComputers in Industry, Volume 49, Issue 3, December 2002, Pages 283-298L. Hadellis, S. Koubias, Vassilios Makios ?Show preview??|?? Related articles??|??Related reference work articles???? Purchase $ 37.95 43 FociCounter: A freely available PC programme for quantitative and qualitative analysis of gamma-H2AX foci??Original Research ArticleMutation Research/Genetic Toxicology and Environmental Mutagenesis, Volume 696, Issue 1, 1 February 2010, Pages 16-20Anna Jucha, Aneta Wegierek-Ciuk, Zbigniew Koza, Halina Lisowska, Andrzej Wojcik, Maria Wojewodzka, Anna Lankoff ?Close preview??|?? Related articles??|??Related reference work articles???? AbstractAbstract | Figures/TablesFigures/Tables | ReferencesReferences Abstract Gamma-H2AX foci are sensitive and specific indicator for the induction of DNA double-strand breaks (DSBs) and an immunocytochemical assay with antibodies recognizing gamma-H2AX has become the gold standard for the detection of this type of DNA lesion. Quantification of gamma-H2AX foci can be achieved by various methods such as Western blotting, flow cytometry, visual analysis and computational analysis with a fluorescence microscope. The best sensitivity is achieved by computer analysis. Since no freeware programme for the analysis of gamma-H2AX foci exists for a PC platform, the aim of our study was to develop a simple and user-friendly public-domain software. The algorithm applied in our programme allows determination of the number of foci in a single cell, a focus intensity per cell, as well as a cell intensity. Its graphical user interface is based on a GTK+ library and the whole application can be run under a variety of operating systems, including MS Windows and Linux. The programme called FociCounter is publicly available at . Application of the programme was tested by analysing gamma-H