Endothelialcells(HUVECs)exposedto25mmolL(25mM).pptVIP

Endothelial cells HUVECs exposed to 25 mmol/L 25 mM glucose compared with 5 mmol/L [5 mM] glucose showed increased FN mRNA A and reduced miR-146a expression B . Biao Feng et al. Diabetes 2011;60:2975-2984 ?2011 by American Diabetes Association Endothelial cells HUVECs exposed to 25 mmol/L 25 mM glucose compared with 5 mmol/L [5 mM] glucose showed increased FN mRNA A and reduced miR-146a expression B . Such changes were not seen when the cells were incubated with 25 mmol/L l-glucose osmotic control [Osm] . Transfection of endothelial cells with miR-146a mimics but not the scrambled mimics reduced basal FN expression and normalized glucose-induced upregulation of FN mRNA A and FN protein C . A: The glucose-like effect FN upregulation was further seen when cells in 5 mmol/L glucose were transfected with miR-146a antagomir. B: The efficiency of miR-146a mimic transfection by increased miR-146a expression following miR-146a mimic transfection compared with scrambled mimics. Likewise, BRMECs showed glucose-induced miR-146a downregulation D and FN upregulation E . E: Transfection of BRMECs with miR-146a mimics reduced basal FN expression and also normalized HG-induced upregulation of FN mRNA. D: The efficiency of miR-146a mimic transfection by increased miR-146a expression following miR-146a mimic transfection compared with scrambled mimics. 146a, miR-146a mimic; 146a A , miR-146a antagomir; S, scrambled miRNA. *Significantly different from 5 mmol/L glucose; **significantly different from 25 mmol/L glucose, miRNA levels are expressed as a ratio of RNU6B U6 ; mRNA levels are expressed as a ratio to β-actin and normalized to 5 mmol/L glucose.