前列腺癌论文：腺素β、β15在前列腺癌组织中表达及意义.doc

前列腺癌论文：胸腺素β、β15在前列腺癌组织中表达及意义 【中文摘要】通过探讨胸腺素β4和胸腺素β15在前列腺癌组织细胞中的表达及其与患者生存时间的关系,以及两者在前列腺癌组织中表达的相关性,以研究及对二者表达与前列腺癌分化、转移的关系进行分析,并探讨其在前列腺癌转移潜能和患者预后评估中的作用及意义。方法实验选取河北大学附属医院泌尿手术切除及前列腺穿刺活检取得的40例前列腺癌组织标本,经过4%多聚甲醛固定,常规脱水,石蜡包埋；每例标本以4μm厚度连续切片,作HE染色确定病理组织类型；用免疫组化S-P法分别检测前列腺癌细胞中胸腺素β4和胸腺素β15的表达情况；所有统计分析用SPSS17.0 for Windows软件进行,采用卡方检验、SPearman等级相关进行分析。结果胸腺素β4蛋白表达在前列腺癌及良性前列腺增生组织的差异有显著意义 χ2 45.039,P 0.01 。胸腺素β15蛋白表达在前列腺癌及良性前列腺增生组织的差异有显著意义 χ2 93.103,P 0.01 。其中免疫组织化学检测胸腺素β4、β15有着较好的敏感度和准确度,分别为70.4% 38/54 、81.5% 88/108 ；92.6 50/54 ,96.3 104/108 ,与常规病理学58% 33/56 、77% 77/100 比较,具有显著性差异 P 0.05 。胸腺素β4蛋白在无淋巴结转移组的阳性率为0% 0/4 ,在有淋巴结转移组的阳性率为76.00% 38/50 ,胸腺素β4蛋白表达在两组间差异有统计学意义 χ2 10.26,P 0.01 。胸腺素β15蛋白在无淋巴结转移组的阳性率为25% 1/4 ,在有淋巴结转移组的阳性率为98% 49/50 ,胸腺素β15蛋白表达在两组间差异有意义 χ2 28.777,P 0.01 。结论胸腺素β4、β15的表达是判断前列腺癌以及是否有无转移的重要指标；胸腺素β4、β15的表达也对患者预后及生存期具有一定的评价意义；胸腺素β4、β15的表达是对患者临床治疗及手术方式的选择具有指导意义。 【英文摘要】:Through discussing the relationship of Thymosinβ4 and Thymosinβ15 in humans prostate cancer cell with its survival time. The relevance of their expression in prostate cancer organizations is also researched in this study. In closed study, the convey form of these two kinds Thymosin Extrasins whether have relevance in prostate cancer cell differentiation and conversion in analyzing these two Thymosin Extrasins. The aim of these actions is to explore its potential in prostate cancer metastasis and prognosis of patients with the role and significance.Methods:The samlple were from Experimental Urology Hospital of Hebei University, selected surgical excision and biopsy of prostate obtained 40 cases of prostate cancer organization, after 4% paraformaldehyde, conventional dehydration, embedded in paraffin; specimens to 4μm thickness of each serial sections for HE staining determine the pathological types, through immunohistochemistry SP method and detected prostate cancer in thymosinβ4 and thymosinβ15 expression; all statistical analysis with SPSS17.0 for Windows software, using chi-square test, SPearman rank correl