一株利用乳酸产丁酸梭菌bey8的鉴定及其特性.docVIP

一株利用乳酸产丁酸梭菌BEY8的鉴定及其特性 胡小红1，周 艳1，陶 勇2*，朱晓宇2，魏 娜2，梁 程2，何晓红2，高 平1* （1.四川大学生命科学学院，成都 610064； 2.中国科学院环境与应用微生物重点实验室，成都 610041） 摘 要：BEY8，基于16S rRNA基因序列的系统发育分析显示， BEY8应为梭菌属（Clostridium），与酪丁酸梭菌模式株DSM 2637 Clostridium tyrobutyricum DSM 2637 有99.8％. BEY8 菌体生长和代谢乳酸的最适pH为5.5-6.0，当pH过低（pH4.8）或过高（pH6.5）菌体均出现明显的生长和代谢延滞，但最终仍能完全将乳酸转化为丁酸；此外，当乳酸浓度达到156mM时，BEY8的生长及代谢同样出现延滞，且延滞时间随乳酸浓度的升高而延长，但最终都能完全将乳酸转化为丁酸，这些特性表明该菌有较强的环境适应能力及代谢稳定性. 在pH4.8时，外加乙酸可显著缩短BEY8的生长延滞期，并提高其对乳酸的代谢速率，但乙酸不能作为菌体的唯一能源生长，而只作为电子受体参与代谢乳酸产丁酸. 关键词：乳酸;pH;酪丁酸梭菌;丁酸;转化率 中图分类号：Q93 文献标志码：A 文章编号： Identification and Characterization of a Clostridium BEY8 to Produce Butyrate from Lactate HU Xiao-Hong1, ZHOU Yan1, ZHU Xiao-Yu2, GAO Ping1*, LIANG Cheng2, HE Xiao-Hong2, TAO Yong2 1. Collge of Life Sciences, Sichuan University, Chengdu 610064, China; 2. Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu 610041, China Abstract: A lactate-utilizing bacterium BEY8 was isolated from a pit mud and it could produce butyrate from lactate. Based on phylogenetic analysis of 16S rRNA gene sequences, the strain BEY8 should belong to the genus Clostridium, and the most closely related type strain is Clostridium tyrobutyricum DSM 2637, with 99.8％gene similarity. The optimal pH values for BEY8 growth and lactate metabolism were found to be 5.5-6.0, and the lag time was observed at pH 4.8 and pH6.5, while lactate could be finally converted into butyrate as the main metabolic product. In addition, when the concentration of lactate was greater than 156 mM, the lag time was also observed, and the arrearage time prolonged with lactate concentration, but finally lactate could mainly be converted into butyrate as metabolic product. It was suggested that BEY8 has a potential to adapt to environment change, and the environmental factors do not affect the change of bacterial metabolic pathway. Furthermore, at pH4.8, the addition of acetate could significantly reduce the lag time of BEY8 growth and improve the me