酸枣仁皂苷论文改善睡眠功能性饮料酒的开发.docVIP

酸枣仁皂苷论文：改善睡眠功能性饮料酒的开发 【中文摘要】睡眠是我们日常生活中一种重要的生理现象,睡眠跟人们的生理和心理活动有密切的关系,对人的健康和生活有着至关重要的影响。良好的睡眠可以帮助人们恢复体力和精力,因此失眠和睡眠障碍问题越来越受到人们的关注。但是,随着现代社会的快速发展,人们的生活节奏也逐渐加快,工作压力以及各方面的压力也越来越大,失眠问题已经成为困扰我们日常生活的常见疾病。我国是使用中草药的大国,中医学对改善睡眠的认识有独到的见解。中医临床主要通过辨证施治,应用中药复方来治疗失眠。目前,有越来越多的学者正在研究中药治疗失眠的病理机理,中草药具有实践的独特疗效性,日益受到世界各国的关注。本论文研究了改善睡眠药酒的开发。卫生部将酸枣仁列为药食同源类物品,根据酸枣仁中有效活性成分酸枣仁皂苷的物理和化学性质,设计提取工艺,并根据Box-Behnken的中心组合设计原理,采用三因素三水平的响应面分析方法,然后对各因子显著性和交互作用进行分析,得出了超声提取酸枣仁皂苷A的最佳工艺条件为:乙醇浓度69.8%,超声温度44.6℃,超声功率74.1w,及此时酸枣仁皂苷A的实际产量为321.09μg/g。采用HPLC对酸枣仁皂苷进行检测。流动相为梯度洗脱:最初5 min为乙腈-水(20:80),5~20 min内乙腈浓度梯度上升为70%,20~22 min乙腈浓度梯度回到20%,检测波长:203 nm。流动相应用梯度洗脱,能将目标物质和其他组分分离开,检测准确。通过对小鼠自主活动影响实验、直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验,研究表明,酸枣仁皂苷具有改善小鼠睡眠的作用。选择红枣、百合、柏子仁、蜂蜜等药食同源的原料进行水提,与超声提取的酸枣仁皂苷进行配伍实验,并以优质黄酒作为酒基,开发了一种低度、营养、具有改善睡眠功能的黄酒饮料。然后进行第二次小鼠药理活性实验,建立一个新型药物模型即酸枣仁皂苷提取物与红枣、百合、柏子仁水提物配伍混合治疗,具有较好的改善睡眠效果。 【英文摘要】The sleep is one of the important physiological phenomenons in our daily life; it closely related to our physical and mental activity, it has an important effect on people’s health and life. Good sleep will make people refreshed, so more and more people have drawn attention to the problem of sleeping quality and insomnia. However, with the rapid development of the modern society, the pace of people’s life become more and more increasingly, the working pressure and others’become larger. As a common disease, Insomnia bothers us in daily life.Chinese herbal medicine is a nation’s treasure and there is unique recognition on improve sleep and insomnia in traditional Chinese medicine. According to the clinical data observed by the Traditional Chinese Medicine, it treats mainly sleep-disorder somnipathy with Chinese medical prescriptions. Currently, more and more scholars are studying path mechanism of insomnia treatment by Chinese herbs. It attracts attention from the entire world for its effect in clinical practice that there is unique curative effect on improving sleep quality with the Traditional Chinese medicines. In t