超声波辅助提取多糖及其抗氧化活性的研究.docVIP

超声波辅助提取大枣多糖及其抗氧化活性的研究 摘 要 大枣多糖是活性多糖之一。近年来，许多学者围绕着大枣多糖的提取分离结构鉴定药理等方面也展开了研究。而大枣多糖的提取方法是首要问题，一种经济有效的提取方法是我们一直探索和研究的。为此，本课题研究了大枣多糖的提取工艺，为大枣多糖进一步的研究与开发奠定基础。 超声波是频率大于20 kHz的声波，具有波动与能量的双重属性。其振动产生并传递强大的能量给予媒质粒子极大的速度和加速度，加速度随着声波频率的增高而增大。超声波在某些物质中可以比一些电磁波穿透得更深，停留时间也较长，足以使物质中分子加速运动。所以近几年，超声波技术在功效物质的提取方面得到了广泛的重视。基于这个理论，采用了超声波辅助法提取大枣多糖，优化了大枣多糖的超声提取工艺。 本试验通过探讨大枣多糖提取的工艺条件，采用单因素试验和L9(34)正交试验设计，研究了不同超声强度、超声时间、pH和料液比等单因素对大枣多糖提取率的影响并优化了提取工艺。采用水杨酸法检测羟基自由基 (·OH)，改进的邻苯三酚自氧化法检测超氧自由基(O2-·)，研究了大枣多糖清除·OH和O2-·的效果。结果表明，最佳提取条件为：1:40的料液比，在超声功率144 W，pH 5.4，超声条件下提取20 min。抗氧化活性试验表明大枣粗多糖对·OH具有较好的清除效果，对O2-·有部分清除作用。 关键词：大枣多糖，超声波，提取，抗氧化活性 Extraction of Jujube Polysaccharide by Ultrasound Method and Its Antioxidant Research ABSTRACT Jujube polysaccharide is one of active polysaccharide. In recent years, many scholars have carried on much study around the extraction and separation of jujube polysaccharide in addition of identifying it’s structure and pharmacology. While the method of extract jujube polysaccharide is the primary problem, we have been exploring and studying an economic and effective method of extract jujube polysaccharide. So we study the extraction of jujube polysaccharide and hope to find the theories to improve its research and application. The frequency of ultrasonic sound waves is greater than 20 kHz, with fluctuations in the dual property and energy. The ultrasonic sound waves can generate and transmit the vibration energy of the powerful media particles to give great speed and acceleration, acceleration with the higher sound frequency increases. Ultrasound in some substances can penetrate deeper than some electromagnetic waves, and longer residence time, sufficient to accelerate the movement of molecular substances. Therefore, in recent years, the efficacy of ultrasound technology in the extraction of material has been widely attention. Based on this theory, the paper adopt the ultrasonic extract jujube polysaccharide and optimize the extraction. In this paper