不同萃取条件对石蓴萃取物抗氧化力的影响.docVIP

不同萃取條件對石蓴萃取物抗氧化力的影響 The effect of extraction methods on the antioxidant capacity of Ulva lactuca extracts 研究生：王聖硯 Wang, Sheng-Yan 指導教授：劉展冏 Liu, Chan-Chiung 【摘要】 過量的活性氧 ROS 會造成細胞損傷，甚至導致癌症、神經退化、老化和發炎。所以許多最近的研究都特別注意天然抗氧化劑。其中主要是酚類化合物，用作膳食補充劑天然食品防腐劑藥品。石蓴 Ulva lactuca 屬綠藻綱 Chlorophyceae ，石蓴目 Ulvales ，石蓴科 Ulvaceae ，在台灣東北角全年均可見，2月至4月為盛產期為了探討石蓴萃取物的抗氧化性及成分，先進行不同方法的萃取。以三種不同萃取溶劑 乙醇、水、醋酸 ，以及不同溫度條件 30、40、50、60 ℃ 下，測定其抗氧化活性 DPPH自由基清除能力、螯合亞鐵離子能力、還原力 總酚、硫酸根、葉綠素、Lab值及FTIR 圖譜。三種抗氧化測定結果皆顯示乙醇萃取物的抗氧化最佳，最佳萃取溫度為40-50℃之間。在DPPH自由基清除能力方面，50℃的乙醇萃取物在1000 ppm下的效果最佳，其自由基清除能力可達90.74%，同濃度的BHA 95.21% 和生育醇α-tocopherol 94.82% 。螯合亞鐵離子能力則為40℃的乙醇萃取物在1000 ppm下的效果最佳 41.05% EDTA在1000 ppm下達到99.96%。還原力方面則為50℃的乙醇萃取物在1000 ppm下的效果最佳 吸光值0.306 低於同濃度的BHA跟生育醇，但卻高於抗壞血酸。總酚和葉綠素含量50℃乙醇萃取物含量最多 分別是42.53 mg/g 和23.48 mg/g 。Lab值方面，三種萃取物皆隨著萃取溫度上升而亮度 L值 增加a值上升b值下降，推測是由於其中色素 主要為葉綠素 受溫度影響而遭破壞，並產生黃色衍生物所導致。硫酸根含量則以60℃醋酸萃取物最高 62.49 mg/g ，50℃水萃物其次 58.42 mg/g ，並與FTIR圖譜檢視官能基。石蓴、綠藻、抗氧化活性、萃取、硫酸根、葉綠素Excessive levels of reactive oxygen species ROS will cause cell damage, eventually lead to the development for a variety of diseases, including cancer, neurodegenerative diseases, aging and inflammation. Many recent researches focused on natural antioxidants, especially natural plants abundant with phenolic compounds, being used primarily as dietary supplements, natural preservatives, and medicines. Ulva lactuca belongs to Chlorophyceae class, Ulvales order, Ulvaceae family, and Ulva genus. It is commonly seen in the northeastern coast of Taiwan, Febuary to April is the peak production season. Ulva is currently a underutilized marine material. Modern medical researches conferred that ulva holds several health benefits to human body, and has the potential for more applications.In order to explore the correlation between the antioxidant capacities and the chemical contents of ulva, three solvents ethanol, water, and 1 N acetic acid aqueous solution , along with different temperatures 30, 40, 50, 60℃ were employed for the extraction of U