基于全基因组信息鉴定中国荷斯坦牛产奶性状基因及 .doc

发表于：畜牧兽医学报, 43（）：2012. 基于全基因组信息鉴定中国荷斯坦牛产奶性状基因及功能注释 齐 超1(，谢 岩1(，吴晓平1，姜 力1，刘剑锋1，孙东晓1*，张 勤1，张 沅1 （1中国农业大学动物科技学院，北京 100193） 摘要本mRNA转录调控。（结论）该研究为进一步鉴定中国荷斯坦牛产奶性状主效基因及功能验证打下了基础。 关键词：中国荷斯坦牛；全基因组关联分析；产奶性状；功能注释 中图分类号： 文献标识码：A 文章编号： Identification and Annotation of Genes Affecting Milk Production Traits in Chinese Holstein Based on GWAS Abstract: (Objective) Our previous genome-wide association studies (GWAS) with Illumina Bovine50K chip have identified 35 significant SNPs associated with one or multiple milk production traits in a Chinese Holstein population from Beijing region with daughter design. The purpose of this study was to determine potential genes affecting milk yield and milk composition and function annotation based on such GWAS results. (Methods) Through bioinformation and comparative analysis, potential genes and function prediction were performed. (Results) We found that 12 of 35 significant SNPs were located within genes and 23 were in the flanking region based on Btau 4.2 database. As a result, total 28 genes corresponding to the aforementioned 35 SNPs were determined. The functions of such genes were classified into 7 categories, including regulate body metabolism and nutrient balance; cytoskeleton or extracellular matrix, regulation of proliferation, cell cycle and apoptosis; cell signal 基金项目： 国家自然科学基金、国家科技支撑项目（2011BAD28B02）、农业部948项目（2011-G2A）、转基因生物新品种培育重大专项（2009ZX08009-156B）lssqc@163.com； 谢岩（1985-)，女，河北兴隆县人，硕士，Tel：010Email: xiey.300822@。 *通讯作者：孙东晓，女，教授，博导， E-mail：sundx@。 transduction and saltion channel composition; kinase activity; mRNA transcription and translation regulation. (Conclusion) Our findings could be useful for further investigations on gene identification and function validation for milk production traits in dairy cattle. Key words：GWAS; milk product trait; function annotation 模拟研究证明实施标记辅助选择以进行青年公牛预选可加快奶牛产奶性状遗传进展[1]，但标记辅助选择的前提是产奶性状主效基因的挖掘和鉴定。过去十几年中，产奶性状QTL（quantitative trait locus）29条常染色