基本资料doc.doc

◎ 醫研部細胞庫常用細胞株細胞及資料來源：食品工業發展研究所生物資源保存及研究中心（BCRC） 第1頁，共頁 Cell line BCRC No. Species Tissue medium note 293 60019 human embryonic kidney 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% Fetal bovine serum Biosafety level：2 生長較慢，且放置室溫時會detached。 3A-sub-E 60302 human placenta;SV40 transformed 90% Minimum essential medium (Eagle) in Earles BSS with 0.1mM non-essential amino acids and 1.0mM sodium pyruvate + 10% fetal bovine serum Biosafety level：2 A549 60074 human lung carcinoma 90% Hams F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate + 10% fetal bovine serum AGS 60102 human gastric carcinoma 90% Hams F-12K medium + 10% fetal bovine serum BCE C/D-1b 60044 bovine eye; cornea; endothelial 90% Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum BNL CL.2 60180 mouse Liver; normal Dulbeccos modified Eagles medium with 4.5 g/L glucose,90%;fetal bovine serum,10%. ◎ 醫研部細胞庫常用細胞株【細胞及資料來源：食品工業發展研究所生物資源保存及研究中心（BCRC） 第頁，共頁 Cell line BCRC No. Species Tissue medium note BT-474 60359 Human breast; mammary gland; ductal carcinoma 90% Hybri-Care Medium (Modified Dulbeccos medium) with 30 ng/ml EGF+10% fetal bovine serum Ca Ski 60251 human cervical epidermoid carcinoma 90% RMPI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose , 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum Biosafety level：2 細胞含Integrated HPV-16 genome，操作培養時應注意HPV感染。 CHO-K1 60006 (hamster, Chinese Ovary 90% Hams F-12 medium + 10% fetal bovine serum COLO 205 60054 human colon adenocarcinoma （heat-inactivated）90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate+ 10%