碳氮同位素在牛肉产地溯源中的应用研究.doc

碳、氮同位素在牛肉产地溯源中的应用研究 郭波莉，魏益民，潘家荣，李 勇 （中国农业科学院农产品加工研究所/农业部农业核技术与农产品加工重点实验室，北京 100094） 摘要：【目的】探索稳定性同位素对中国牛肉产地溯源的可行性，了解牛不同组织中δ13C和δ15N指标在其中的作用。【方法】利用同位素比率质谱仪测定了来自吉林、贵州、宁夏、河北4个地域牛肉、牛尾毛、饲料中的δ13C和δ15N值，比较了不同地域牛组织中稳定性碳、氮同位素组成的差异，分析了各组织中同位素组成的相关关系，并结合牛的喂养方式，研究了δ13C和δ15N值的变化规律。【结果】不同地域牛组织中稳定性碳同位素组成有极显著差异，排序依次为吉林贵州宁夏河北。吉林、宁夏两地样品与贵州、河北两地样品之间的δ15N值有极显著差异，且后者高于前者。不同地域喂养牛的主饲料成分有其明显的地域特征。脱脂牛肉粉、粗脂肪、牛尾毛中碳、氮同位素之间呈极显著相关性。【结论】牛组织中δ13C值可预测其膳食中C4植物所占的比例，δ15N值可区分牧区与农区喂养的牛；δ13C指标对牛肉产地来源的正确判别率高于δ15N指标，两项指标相互补充可显著提高产地来源的正确判别率；脱脂牛肉粉、粗脂肪、牛尾毛中δ13C、δ15N值均可作为牛肉产地溯源的指标，它们对牛肉产地来源的判别规律一致。 关键词：同位素；溯源；牛肉；产地；δ13C；δ15N Application of Carbon and Nitrogen Isotope in Beef Origin Traceability GUO Bo-li, WEI Yi-min, PAN Jia-rong, LI Yong (Key Laboratory of Agriculture Nuclear Technology and Agro-Food Processing of Ministry of Agriculture, Institute of Agro-Food Science Technology, Chinese Academy of Agricultural Sciences, Beijing 100094) Abstract：【Objective】To assess the feasibility of isotopic traceability and function of δ13C and δ15N values in beef geographical traceability in China 【Method】The δ13C and δ15N values of beef, cattle hair and feeds collected from Jilin, Guizhou, Ningxia and Hebei were investigated with an isotope ratio spectrometer, the difference of carbon and nitrogen composition of beef samples from above four regions were compared and the correlations of isotope composition between cattle tissues were analyzed, furthermore, change of δ13C and δ15N values of beef samples under different feeding modes were also researched. 【Result】The δ13C values of cattle tissues from different regions showed significant difference in the order of JilinGuizhouNinxiaHebei. The δ15N values of cattle tissues from Jilin and Ningxia were greatly significant lower than that from Guizhou and Hebei. The staple feeds from different regions had their own character. In addition, significant correlation was found in δ13C and δ15N values between defatted muscle, crude fat and cattle hair. 【Concl