药用桃金娘科植物萃取物对胰岛素阻抗小鼠肝细胞FL83B葡萄糖摄入.docVIP

藥用桃金孃科植物萃取物對胰島素鼠細胞ffects of the extracts from pharmaceutical Myrtaceae plants on the glucose uptake in insulin-resistant mouse FL83B cells 研究生： Jia-Woei Chang 指導教授：、 Mei-Li Wu , Ming-Chang Wu 【摘要糖尿病是現今常見的一種慢性代謝疾病，已被世界衛生組織列為第三個最常發生的慢性疾病。番石榴、肯氏蒲桃等為食用性桃金孃科植物，於亞洲和南美洲常被用於治療糖尿病指出，番石榴葉水萃和醇萃物對於糖尿病鼠具顯著降血漿中葡萄糖濃的；E. jambolana種子水萃和醇萃物葡萄糖耐受性試驗結果顯示對於糖尿病兔有降血糖之功能。藥桃金孃科植物檸檬按 Eucalyptus citriodora 和馬奴卡 Leptosperum scoporium 葉萃出之精油具抗菌、抗發炎等作用；稜果蒲桃 Eugenia uniflora 葉有降血糖之效果；台灣赤楠 Syzygium formosanum 葉具有強的抗氧化活性。流式細胞儀檢測2-NBDG攝入細胞的方式FL83B細胞株以不添加D-glucose之KRB緩衝溶液懸浮，並與200 μM 2-NBDG0 分鐘，以流式細胞儀可偵測到最大螢光強度。用20 ng/mLTNF-α誘導FL83B細胞具胰島素阻抗胰島素阻抗。使用四種藥用桃金孃科植物：檸檬按、台灣赤楠、稜果蒲桃和馬奴卡，並以胰島素阻抗細胞模式篩選出具有降血糖之植物。四種藥用桃金孃科植物葉之水和乙醇萃取物進行FL83B細胞存活率試驗，結果發現檸檬按葉粗萃物 800 μg/mL 對細胞無毒性，台灣赤楠、稜果蒲桃和馬奴卡葉之粗萃物於高濃度800 μg/mL皆對細胞有毒性。四種樣品粗萃物處理TNF-α誘導FL83B細胞後，結果顯示，50 μg/mL台灣赤楠葉粗萃物對具胰島素阻抗之FL83B細胞會明顯改善葡萄糖攝入情形，且與胰島素處理 之正控制組效果相當。關鍵字：糖尿病、桃金孃科、降血糖、2-NBDGAbstract】 Diabetes mellitus DM is one of the major diseases that human being eagerly wants to overcome and has been listed as the third most commonly occurred chronic disease by WHO. In Asia and South America, the leaves and fruits from some Myrtaceae plants such as Psidium guajava and Eugenia jambolana may have the potential in curing diabetes. The report pointed out that the water and ethanol extracts of P. guajava leaves significant decreased the plasma glucose level of diabetic rats. The water or ethanol extracts of E. jambolana seeds exhibited antihyperglycaemic effect in diabetic rabbits during glucose tolerance test. Essential oils extracted from Pharmaceutical Myrtaceae plants, such as Eucalyptus citriodora and Leptosperum scoporium, leaves had antiseptic and anti-inflammatory effects. Eugenia uniflora leaves showed anti-hyperglycemic effect. It was reported that leaves of Syzygium formosanum also had the excellent antioxidant activities. The present study aimed to investigate the anti-hyperglycemia effect of pharmaceutical Myrtaceae plants. An insulin resistant cell model was est