Chapter1Introduction资料.ppt

Total 64 Pages Bioseparations Engineering Chapter 1 Introduction 1.1 What is Bioseparation 1.2 The characteristics of Bioseparation 1.3 The unit operations in Bioseparation 1.4 Technical Examples in Bioseperation 1.5 Evaluation of Bioseparation 1.6 The Objective of the Course What is Bioseparation The characteristics of Bioseparation Bioproduction can be broadly classified into the following categories: Small molecules, consisting of fine chemicals, antibiotics, hormones, amino acids, and vitamins Large molecules, consisting of proteins, polysaccharides, and nucleic acids, and particulate products, consisting of cells, spores, liposomes, and subcellular particles or organelles Small Biomolecules Small biomolecules can be divided into two categories: primary metabolites and secondary metabolites. Primary metabolites including: Sugar, Organic alcohols acids and ketones, Vitamins, Amino Acids, is one that is formed during the primary growth phase of the organism. Secondary metabolites including: antibiotics are produced at or near the beginning of the stationary phase. Small Biomolecules Nucleic acids and Oligonucleotides Nucleic acids and Oligonucleotides Polysaccharides Technical Examples in Bioseperation Example: p33 You have been given the assignment to purify the antibiotic erythromycin 红霉素 . You have at your disposal the Merck Index, which has the information on erythromycin shown in Figure E1.2. What do you think are the most likely unit operations that should be used for the isolation and purification of erythromycin? Solution: Evaluation of Bioseparation Evaluation of Bioseparation Period distribution Evaluation Homework class attendance: 15% Presentation: 15% Final written examination: 70% ★ Poor stability 稳定性差 Due to chemical degradation or microbial degradation, the products can be inactivated by temperature, pH or various enzymes. ★ High purity and strict sanitation criterion Almost all products are used as medicine, biological reag