ImmunofluorescencestainingofCD34-selectedputativeEPC.pptVIP

Immunofluorescence staining of CD34-selected putative EPC from CDNAC after 4 weeks of culture. Juliane Eggermann et al. Cardiovasc Res 2003;58:478-486 Copyright ? 2003, European Society of Cardiology Immunofluorescence staining of CD34-selected putative EPC from CDNAC after 4 weeks of culture. Immunofluorescence staining was performed after fixation of cultured cells on day 28. Cells were labelled with a PE-conjugated antibody recognising CD133 A and with unconjugated antibodies against VEGFR-2 B and von-Willebrand-factor C , which were detected by FITC-phalloidin. Cells were counterstai