EpCAMundergoesRIP(A–D)SizedeterminationofEpCAM.pptVIP

EpCAM undergoes RIP A–D Size determination of EpCAM by Western blot. Ulrike Schnell et al. Biosci. Rep. 2013;33:e00030 ?2013 by Portland Press Ltd EpCAM undergoes RIP A–D Size determination of EpCAM by Western blot. A Lysates LYS and concentrated medium MED from HT29 cells were analysed with antibodies against EpCAMs ECD or ICD as indicated. B HT29 lysate, concentrated medium before and after ultracentrifugation 100000?g for 1?h , and the pelleted fraction resulting from ultracentrifugation were immunoblotted against EpCAMs ectodomain 311-1K2 . C Distinct ratios of EpEX and Ep