地黄饮片HPCE-DAD指纹图谱的(中药材)概要.docVIP

地黄饮片HPCE-DAD指纹图谱的研究 摘要 目的：建立地黄饮片HPCE-DAD指纹图谱分析方法，并对地黄及其炮制品的指纹谱进行比较。方法：采用毛细管电泳法进行色谱分离，以60mmol·L –1硼砂 (5%甲醇，Ph 9.5)为运行缓冲液，分离电压为20 kV，波长为210nm，以梓醇为参照物（IS），测定其指纹图谱，并作模糊聚类法分析和相似度评价。结果：初步建立了以个共有峰为特征指纹信息的HPCE-DAD指纹图谱；发现少数地黄饮片的指纹图谱有一定差异，生品与其炮制品的指纹谱中共有峰相对峰面积差异显著。结论：方法准确可靠，重现性好，可作为黄饮片内在质量评价的依据。 关键词 地黄；二极管阵列检测法；指纹图谱Studies on HPCE-DAD fingerprint of Rehmannia glutinosa Xu Hu , HAN Le , LIU Xunhong*, FU Xingsheng, LI Junsong , CAI Baochang，SHEN Zezhong (Nanjing University of Chinese Medicine, Nangjing 210046, China) Abstract Objective: To establish the analytical method for the fingerprint of Rehmannia glutinosa by HPCE-DAD and compare the fingerprints of Rehmannia glutinosa and its processed products. Method: Based on the mode of high performance capillary electrophoresis，60mmol·L - 1 sodium borate was used as buffer solution (5%MeOH, pH9.5). The separation voltage was kV and the detection wavelength was set at 210 nm. Catalpol was used as a reference standard，the chromatographic fingerprint were determined. The data were analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples. Result: -DAD fingerprints with 7 common peaks of Rehmannia glutinosa were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Rehmannia glutinosa and its processed products，there were obvious differences in the relative areas of common peaks. Conclusion: The method is reliable， accurate and can be used for quality control of Rehmannia glutinosa. Key words Rehmannia glutinosa；-DAD；fingerprint地黄为地黄科植物地黄Rehmannia glutinosa Libosch..的块根，具有清热凉血、养阴、生津之功效，始载于《神农本草经》，列为上品，为我国“四大怀药”之一。《中国药典》2010版收载有鲜地黄、生地黄、熟地黄，功效各不相同。鲜地黄凉血清火、生津止渴，生地黄清热凉血、滋阴生津，熟地黄补血、滋阴[]。临床上也有用生地炭、熟地炭，生地炭长于凉血止血，熟地炭长于补血止血[2]。[3]。其主要有效成分为环烯醚萜类化合物。目前对地黄的质量控制，多以梓醇等作为考察指标[4-5]，这种[6]。本文采用高效毛细管电泳法对地黄饮片指纹图谱的构建进行了探讨，并与其炮制品进行比较分析，以期为规范地黄饮片市场及确保药材内在质量的均一性和稳定性提供较全面的质量控制手段。 1 仪器与材料 G1600-AX高效毛细管电泳仪（美国Agilent公司），配惠普化学工