GPC5水溶性GPC解析.ppt

26198 Water Soluble Polymers Rev. 21199 水溶性聚合物Water-Soluble Polymers Objectives for Water-Soluble Polymers To show how aqueous based SEC is different from organic based SEC. Show how to operate under the differences and be successful Water Soluble Polymers Water-Soluble Polymers Non-Size-Exclusion Effects in GPC of Water-Soluble Polymers Intramolecular Electrostatic Attractions Ion Exclusion Ion Inclusion Ion Inclusion con’t Observations: An additional peak appears at large elution volumes near the penetration limit, decreasing the differential refractometer signal by lowering the salt concentration in the range of the polymer peak. Precautions: Concentration of the added salt as low as possible. Reduction of the polyelectrolyte dissociation by choosing a polymer-specific pH value. Adsorption by Ion Exchange Adsorption in Aqueous GPC Adsorption Effects in Aqueous GPC Adsorption bySolvophobic Interactions Adsorption by Hydrogen Bonding Solvophobic Association Memory Effects in GPC ofPolyvinylamine Eluent Choice Aqueous GPC Eluent Selection for Methacrylate Packings Aqueous GPC Eluent Selection for Methacrylate Packings Capability Review of Aqueous SEC Columns Polymethacrylate Columns Shodex KB Separation Ranges Calibration Curve for Shodex KB 80M 2 + 802.5 Poly Ethylene Oxide Standards Molecular Weight Alginic Acid, Na Salt Distribution Overlays of Three Organic Acids Polyacrylamide, MW 2,200,000 Polysine 聚赖氨酸 References for Aqueous SEC of Polymers Packing materials and experimental conditions for single polymers: A.R. Cooper Developments in Polym. Charact.5 1986 131-177 Non size exclusion effects: H.G.Barth ADV. Chem. Ser. 213 1986 31-55 Obstacles to the SEC of ultrahigh molecular weight samples: J.C. Giddings Adv. Chromatogr. 20 1982 217-258 References cont. Textbook available: Aqueous Size Exclusion Chromatography, J. of Chromatography Library-40 1988 Elsevier Davidson, Robert l., Handbook of water soluble gums and resins 1980 , Mc