植物保护学报2007年第6期文摘.docVIP

植物保护学报2007年第6期文摘 小麦叶锈菌的特异性分子诊断检测技术=Molecular diagnosis and detection of Puccinia triticina in China[刊，中]/ 曹丽华(河南农业大学植物保护学院，郑州450002),徐世昌,陈万权,刘太国,蔺瑞明 //植物保护学报. —2007，34（6）.—561～566 摘要：小麦叶锈病由Puccinia triticina引起，是我国小麦生产上的重要病害。本研究旨在建立小麦叶锈菌快速、准确的PCR诊断检测技术体系，用于病害精准测报和综合防控。以真菌β-微管蛋白基因的保守序列为引物，进行小麦锈菌gDNA的PCR比较分析，发现小麦叶锈菌具有长度为268 bp的特异性DNA片段；序列分析后设计了2对专化性引物，成功获得检测灵敏度为5.00 pg/μL模板DNA浓度水平的小麦叶锈菌种的特异性SCAR标记。对55个来自我国不同麦区的小麦叶锈菌标样以及其它麦类病原真菌的检测表明，该叶锈菌标记的检测可靠性达100％。人工接种条件下，叶锈菌侵染24 h后，即可在小麦叶片内检测到该标记。图6参15 关键词:小麦叶锈菌；SCAR标记；PCR检测 Abstract: Wheat leaf (brown) rust, caused by Puccinia triticina, is one of the important foliar diseases of wheat in the world. Our objective of this research was to develop PCR assays for rapid identification and detection of P. triticina, which would be utilized in the accurate forecast and seasonal control of this destructive disease. The genomic DNA of P. triticina and P. striiformis was comparably amplified by a pair of primers based on conserved β-tubulin gene sequence, and then a 268 bp specific DNA fragment of P. triticina was isolated and purified. Based on its sequence, another two primer sets were designed successfully to obtain the new sequence characterized amplified region (SCAR) markers of P. triticina, which could be amplified in all test isolates of P. triticina, whereas no DNA fragment was obtained in the other alien fungus species. The detection accuracy of the primer sets of BAFBY (f1) /BAFBY(r) was 5.00 pg/μL. The new SCAR markers of P. triticina can also be detected in wheat leaves post -inoculated 24 h. Development of a high-through kit for the rapid diagnosis and detection of wheat leaf rust based on the result would be anticipated in the further study. 15 Refs. In Chinese. Key words: Puccinia triticina; SCAR marker; PCR detection email: caolihua@ 2004—2005年北方部分麦区白粉病菌小种动态及流行相关区品种抗性分析=Analysis on 2004-2005 racial virulence of Blumeria graminis f.sp.tritici in northern China and the resistance in wheat cultivars in the disease epid