Enzyme10.11酶.pptVIP

concentration of enzyme [E] concentration of substrate [S] pH Temperature Inhibitor Activator Km is a characteristic constant of E. The value of Km quantifies the affinity of the enzyme and the substrate under the condition of K3 K2. The larger the Km，the smaller the affinity. the substrate concentration at which enzyme-catalyzed reaction proceeds at one-half of its maximum velocity Km is independent of [E]. It is determined by the structure of E, the substrate and environmental conditions (pH, T, ionic strength, …), Even with the same enzyme, if the substrate is different, Km will be d