人Latexin基因及蛋白质的生物信息学分析_王兆松.docVIP

第 20 卷 第 2 期 生命科学研究 Vol．20 No．2 2016 年 4 月 Life Science Research Apr. 2016 DOI:10.16605/ki.1007-7847.2016.02.006 人 Latexin 基因及蛋白质的生物信息学分析 王兆松，赵文铖，周 蒙，魏 梅，董秋萍，许世磊* （天津医科大学肿瘤医院 国家肿瘤临床医学研究中心 天津市肿瘤防治重点实验室，中国天津 300060） 摘 要: Latexin（Lxn）是人的羧肽酶抑制剂, 并有肿瘤抑制因子的作用。 利用生物信息学分析 Lxn 基因的启动子和 CpG 岛以及 Lxn 蛋白质的理化性质、结构特点、功能特征后发现, Lxn 基因存在两个启动子和 CpG 岛, 且 一个 CpG 岛与启动子重合; Lxn 是全长 222 个氨基酸, 等电点 5.52, 无信号肽、 无跨膜结构的亲水不稳定蛋白 质; 蛋白质二级结构有 4 个 α 螺旋和 9 个 β 折叠, 三级预测结构可信度为 99.55%, 拉曼图表明结构稳定; 与 Lxn 相互作用的蛋白质主要是羧肽酶, 另外 Lxn还参与炎症反应和温度引起的痛觉反应等生物过程; 启动子和 氨基酸在灵长类间的同源性极高。 对 Lxn 的表达、结构及功能的预测分析可以为研究 Lxn 在生命过程中的作 用提供重要的信息。 关键词: Latexin; 羧肽酶抑制剂; 肿瘤抑制因子; 生物信息学 中图分类号：Q811.4 文献标识码：A 文章编号：1007-7847(2016)02-0125-06 Bioinformatic Analysis of Human Latexin Gene and Protein WANG Zhao-song, ZHAO Wen-cheng, ZHOU Meng, WEI Mei, DONG Qiu-ping, XU Shi-lei* (Tianjin Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China) Abstract: Latexin (Lxn) is a carboxypeptidase inhibitor in human and acts as a tumor -inhibiting factor. Through bioinformatic analysis, predictions on the promoter and CpG island of Lxn gene, as well as physico－ chemical properties, structural and functional characteristics of Lxn protein were performed. It was found that there are two promoters and two CpG islands in the gene and one CpG island is located in one promoter region. Lxn protein is composed of 222 amino acids, with isoelectric point of 5.52. It is a hydrophilic and unstable protein without a signal peptide and transmembrane domain. As for the advanced structure, four α-helices and 9 β-sheets were predicted in the secondary structure. The reliability of predicted tertiary struc－ ture reached up to 99.55% and the structure was proven to be stable by Ramachandran plot. The advanced structure influenced the function of protein. Lxn not only interacted with carboxypeptidase but also took par