毕业设计(论文)-环境浓度微囊藻毒素-LR对水稻生态毒性效应研究.doc

提供全套毕业论文图纸，欢迎咨询 本科毕业论文 题 目： 学 院：化学工程学院 专 业：环境工程 班 级: 1002051 学 号：100205119 学生姓名： 指导教师： 职称: 讲师 职称： 二零一四年 五月日摘 要 近年来，我国蓝藻水华暴发分布日趋广泛且严重，大量微囊藻毒素随着农业灌溉用水进入农田，目前对该过程中高等植物对蓝藻毒素的吸收及由此引发的多种毒性效应的机制尚未明确。本实验以水稻为研究对象，采用现代分析技术和分子生态毒理学方法，考察了微囊藻毒素对受试作物的毒性差异，研究了不同浓度微囊藻毒素对水稻生长发育的影响，检测指标包括:1)植株营养学指标如株高、鲜重、根长；2)生理生化指标如过氧化物酶活性、谷胱甘肽含量、丙二醛含量；3)光合作用指标如净光合作用、叶绿素荧光、蒸腾速率等。主要研究结果表现为：微囊藻毒素浓度对株高、等在MC-LR为0.1 ug/L的时候有促进作用，紧接着1ug/L的浓度下就有了明显的抑制作用，浓度50 ug/L的抑制作用极显著；而微囊藻毒素对植株鲜重、丙二醛含量、叶绿素荧光和蒸腾速率则表现为抑制作用，且浓度越高，抑制作用越明显。该研究对阐明。 关键词：微囊藻毒素水稻  Environment concentration microcystin - LR ecological toxicity effect of rice research ABSTRACT In recent years, the distribution of algal bloom increasingly widespread and severe. It cause a large number of blue-green algae toxins entering the crop field along with the agricultural irrigation water. So far people know few about how higher plants absorb these toxins and the resulting toxicity mechanisms. With rice as the research object, we use modern analytic techniques and molecular ecotoxicology method to examine the toxicity of microcystin on a crop of difference and study the different concentration of algal toxins on rice growth and development. Detection plant nutrition indicators, including 1) Plant nutrition indicators such as amylase, plant height, fresh weight, root length, 2) physiological and biochemical indicators such as alkaline phosphatase activity, glutathione, malondialdehyde content, 3) photosynthesis indicators such as net photosynthesis, chlorophyll fluorescence, transpiration rate. The results shows that Microcystin concentration on plant height, fresh weight, with long in MC - LR concentration is 0.1 ug/L has a promoting effect, followed by 1 ug/L concentrations had obvious inhibitory effect, the concentration of 50 ug/L inhibition significantly. And microcystin algae toxins to plant fresh weight, malondialdehyde content, transpiration rate and