酶级联合成己内酯bornscheuer德国应化.pptxVIP

An Enzyme Cascade Synthesis of ?-Caprolactone and its Oligomers严阳Author: Uwe T BornscheuerProfessor, Head of Dept. University of Greifswald · Biotechnology and Enzyme Catalysis Group格赖夫斯瓦尔德大学EDUCATIONEditor-In-Chief of the http://www.wiley-vch.de/publish/en/journals/alphabeticIndex/2114/Eur.Jhttp://www.wiley-vch.de/publish/en/journals/alphabeticIndex/2114/. Lipid Science Technol.Chairmen of Editorial Board of /journal/122208635/homeChemCatChemCo-Editor of the?/R?mpp/-Online?(together with?http://www.biologie.uni-hamburg.de/bzf/fbga031/fbga031.htmProf. http://www.biologie.uni-hamburg.de/bzf/fbga031/fbga031.htmStreit) and responsible for the area Biotechnologie Gentechnik“…AuthorResearch Direction“Our major research target is the development of tailor-made biocatalysts suitable for industrial applications.”Overview of research activities:Access to biocatalystsIdentification of new biocatalysts by screening, e.g. from strain collectionsIn silicodiscovery（电脑模拟探索） of novel enzymesCloning and functional expression in suitable microbial hostsCreation of tailor-made enzymes using directed (molecular) evolution and rational protein designCommercial enzymesUniversityThe?University of Greifswald?(格赖夫斯瓦尔德大学)is a public research university located in Greifswald, Germany, in the state of Mecklenburg-Vorpommern.Founded in 1456 , it is one of the oldest universities in Europe. Due to the small-town atmosphere, the pronounced architectural presence of the almamater across town, and the young, academic flair in the streets, Greifswald is often described as a university with a town built around it rather than a town with a university.InstitutionKey pointsChemical synthesis route: ?-Caprolactam from the Cyclohexanone Oxime Route ε-己内酯的环己酮氧化合成途径Enzyme cascade synthesis route (in this article): from cyclohexanol to oligo-?-Caprolactone by the enzymes：ADH (enzyme 1)+ CHMO (enzyme 2)+ lipase CAL-A (enzyme 3)Chemical synthesis of ?-Caprolactoneonly modest selectivity (85–90%)toxicityecolo