大豆脂肪酸主要组分含量QTL定位.docVIP

1 大豆脂肪酸主要组分含量QTL定位? 邹 筱 韩粉霞? 陈明阳 孙君明 南金平 闫淑荣 杨 华 中国农业科学院作物科学研究所 / 农业部北京大豆生物学重点实验室 / 农作物基因资源与基因改良国家重大科学工程，北京 100081 摘 要：以中黄13×中黄20的100个BC2F2家系为作图群体，构建了一张包含131个SSR分子标记的遗传连锁图谱，图谱 总长为2157.3 cM，平均遗传距离为16.5 cM，涵盖了大豆的20个连锁群。利用气相色谱技术测定BC2F2、BC2F3和BC2F4 回交群体的脂肪酸主要组分含量，采用IciMapping 3.3完备区间作图法定位QTL，共检测到5种脂肪酸组分相关的QTL 26 个，与棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸相关的QTL分别为5、5、7、5和4个；3个区间在不同年份被检测到与同 一脂肪酸组分相关，sat_294~satt228连续3年被检测到与棕榈酸含量相关，sat_253~satt323和sat_292~satt397连续2年被检 测到与油酸含量相关；4个区间被检测到与2种脂肪酸组分相关，其中sat_294~satt228与棕榈酸、油酸相关，satt308~sat_422 与硬脂酸、亚油酸相关，sat_292~satt397与油酸、亚油酸相关，satt374~satt269与油酸、亚麻酸相关。 关键词：大豆；脂肪酸；遗传图谱；QTL Quantitative Trait Loci of Major Fatty Acid Components in Soybean ZOU Xiao, HAN Fen-Xia?, CHEN Ming-Yang, SUN Jun-Ming, NAN Jin-Ping, YAN Shu-Rong, and YANG Hua Institute of Crop Science, Chinese Academy of Agricultural Sciences / Key Laboratory of Soybean Biology (Beijing), Ministry of Agriculture / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China Abstract: The mapping population with 100 BC2F2 lines of soybean backcross from Zhonghuang 13 × Zhonghuang 20 was developed and tested for its genetic variation. A linkage map with a total distance of 2157.3 cM was constructed with an average distance of 16.5 cM using 131 polymorphism SSR markers. Five kinds of fatty acid components were measured by GC in three years. The QTLs of major fatty acid components were analyzed with ICIM by IciMapping 3.3. The results showed that 26 QTLs associated with five fatty acid components were detected, including five QTLs for palmitic acid, five QTLs for stearic acid, seven QTLs for oleic acid, five QTLs for linoleic acid and four QTLs for linolenic acid. There were three intervals related to the same component detected in different years. The interval of sat_294–satt228 related to palmitic acid was detected in three years, the intervals of sat_253–satt323 and sat_292–satt397 related to oleic acid were detected in two yea