加味圣愈汤对大鼠脑外伤后炎症反应的干预和神经保护作用.docVIP

加味圣愈汤对大鼠脑外伤后的神经保护作用＊ 赵广伟1, 2，姜正林1，李永财3，喜新4，马东明3，王勇4，王国华1 （1南通大学航海医学研究所，江苏226001；2南通大学附属医院神经内科，江苏226001； 3宁夏回族自治区人民医院,宁夏750021；4南通市第一人民医院，江苏226001） 摘要: 目的 研究加味圣愈汤治疗对创伤性脑损伤（traumatic brain injury, TBI）大鼠的神经保护作用，并探讨其作用机制。 方法 运用改良Feeney法建立大鼠脑损伤模型，将动物随机分为假手术组、脑外伤组、（0.5，1.0，2.0ml/200gwt）加味圣愈汤治疗组。对伤后大鼠进行神经行为学观察，并采用干湿法测量脑含水量，尼氏染色光镜下观察海马细胞形态，并测量脑组织损伤体积，ELISA法测定脑外伤后72小时脑组织中TNF-α、IL-1β、IL-6、IL-10的表达水平。 结果 与单纯脑外伤组比较，加味圣愈汤治疗可明显改善神经行为，降低脑含水量，减轻海马神经细胞损伤，抑制TNF-α、IL-1β水平的升高与IL-10水平的下降，差异有统计学意义（P0.05），而脑组织损伤体积和IL-6的表达却未见明显改变（P0.05）。结论 加味圣愈汤治疗脑外伤对脑组织有保护作用，其机制可能与调节脑组织中炎症相关因子的表达，从而减轻炎症反应有关。 关键词 : 创伤性脑损伤；加味圣愈汤；神经保护；炎症因子 中图分类号 : R651.1+5 【文献标志码】A 【文章编号】 Neuroprotective effects of modified Shengyu Decoction on traumatic brain injury in rats ZHAO Guang-Wei1, JIANG Zheng-Lin2, LI Yong-Cai3, XI Xin4,MA Dong-Ming3, WANG Yong4, WANG Guo-Hua2 (1Institute of Nautical Medicine, 2Department of Neurology, Affiliated Hospital, Nantong University, Nantong, 226001 China; 3The People’s Hospital of Ningxia, Yinchuan, 750021 China; 4The People’s Hospital of Nantong, Nantong, 226001 China) Abstract :【Objective】 To investigate the neuroprotective effect of modified Shengyu Decoction on traumatic brain injury (TBI) in rats, and the underlying mechanisms. 【Methods】 The modified Feeney’s method was used to establish TBI model in rat. Rats were randomly divided into sham-operated group, TBI group and (0.5, 1.0, 2.0ml/200g) modified Shengyu Decoction -treated groups. Post injury, the neurological functions were observed. The brain water content was measured with wet-dry weight method. The morphological change of neuron in hippocampal area and cerebral contusion volume were observed under optical microscope through Nissl staining. The contents of TNF-α, IL-1β, IL-6, and IL-10 in the brain tissue were detected with ELISA method. 【Results】 After modified Shengyu Decoction treatment, compared with the TBI group, recovery of the neurological functions was promoted (P0.05), the hippocampal injury wa