培训课件_急性白血病诊治进展.pptVIP

* Except NPM1 mutation, the role of other molecular mutations in MRD monitoring is not well studied. * * All mutations occurred as an insertion of four nucleotides at position 960, except for type VII, which had an insertion at position 964. Method: The PCR products were purified and directly cloned into pGEM-T or pGEM-T-EASYvector. The plasmids were sequenced and the wild-type (WT) and mutant clones were used for standard curves in subsequent real-time PCR. The seven mutants were serially diluted from 106 to 10 copies per reaction and found as low as 10 copies of all mutants could be reliabl