BY-2細胞的培养与转化.docVIP

BY-2 Cells: Culture and Transformation for Live Cell Imaging BASIC PROTOCOL 1 ESTABLISHING A BY-2 SUSPENSION CULTURE BY-2悬浮培养的建立 This protocol describes the method to establish a BY-2 cell suspension from a BY-2 or another tobacco callus. Subculturing the cells is technically termed passaging (also see Basic Protocol 2). It is advisable to work in duplicate or even triplicate when establishing a suspension culture. This allows initiation of fresh suspensions of the same transformed cell line should any contamination occur. 这个实验步骤描述了通过BY-2或者其他的烟草愈伤组织建立BY-2细胞悬液的方法。传代培养细胞是一个传代的专有名词。当建立悬浮体系的时候最好是