五福心腦清动脉粥样硬化氧自由基及主动脉ICAM-1表达的影响.doc

五福心脑清对动脉粥样硬化模型大鼠氧自由基 及主动脉ICAM-1表达的影响 郭建民 武警河北总队卫生处 河北 石家庄 050051 【摘 要】目的：研究五福心脑清对大鼠动脉粥样硬化氧自由基及细胞间粘附分子（ICAM-1）表达的影响。方法：应用高脂饲料复制SD大鼠早期动脉粥样硬化模型,分别给以五福心脑清、辛伐他汀片灌胃,实验10周后,全自动生化分析仪检测各组大鼠血脂、血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量，用免疫组化和RT-PCR方法检测主动脉壁ICAM-1及其mRNA的表达水平。结果：两治疗组血脂、血清MDA含量均明显降低, SOD活性显著升高， ICAM-1及其mRNA的表达水平显著下降，且五福心脑清优于辛伐他汀。结论：本实验说明五福心脑清抗动脉粥样硬化作用的机制可能与其抗氧化、抗粘附等保护血管内皮细胞功能密切相关。 关键词：五福心脑清；动脉粥样硬化；氧自由基；细胞间粘附分子 Effects of Wuxinnaoqing on aortic endothelial cell’s oxyradical and the expression of ICAM-1 in a rat model of atherosclerosis GUO Jian-min（Department of Hygiene, Hebei Municipal corps Hospital, People’s Armed Police Forces of China, Shi jiazhuang 050051） [Abstract] Objective: To investigate the effects of Wufuxinnaoqing on the oxyradical and intercellular adhesion molecule (ICAM-1) of aortic endothelial cell of atherosclerosis blood vessel. Methods: The model of SD rat atherosclerosis was induced by cholesterol diet; the suspension of Wufuxinnaoqing and simvastatin were administered intragastrically, respectively. After 10 weeks, the serum lipids, superoxide dismutase (SOD) and maleic dialdehyde (MDA) were detected by automatic biochemistry analyzer. ICAM-1 and its expression of mRNA in aortic wall were detected by immunohistochemistry and RT-PCR, respectively. To observe aortic histomorphology by HE stainning. Results:The results show that the serum lipids, superoxide dismutase (SOD) and maleic dialdehyde (MDA) of treated groups were obviously improved compare with those of the model group; ICAM-1 and its expression of mRNA was decreased obviously, and the effect of Wufuxinnaoqing was better than that of simvastatin. Conclusion: It is suggested the mechanism of anti-atherosclerosis of Wufuxinnaoqing have close relationship with the function of its anti-oxidizing and anti-adhesiveness that can protect aortic endothelial cell. [Key words] Wufuxinnaoqing; Atherosclerosis; oxyradical; ICAM-1 目前，随着生活水平的提高和人口老龄化，心血管疾病发病率呈上升趋势，严重威胁人类健康，有关冠心病和动脉粥样硬化方面的研究已成为临床和基础研究的热点。五福心脑清