海參花水提物对环磷酰胺诱导小鼠生殖系统受损的影响.docVIP

海参花水提物对环磷酰胺诱导小鼠生殖系统受损的影响 摘要：目的 本文旨在探讨海参花水提物对环磷酰胺诱导的小鼠生殖系统受损、卵巢早衰（POF）的保护作用。方法 雌性昆明小鼠随机分组，除空白组每天注射生理盐水外，其余各组连续5天腹腔注射环磷酰胺28（mg/kg），造成小鼠生殖系统紊乱，建立小鼠卵巢早衰模型。放免法检测血清雌二醇含量（E2)、孕酮（P)含量，比较各组小鼠间子宫、卵巢、脾、胸腺等脏器重量，并进行小鼠卵巢、子宫组织形态学观察。结果 连续用药4周后，与模型组相比，海参花水提物各剂量组均能显著增加小鼠的子宫、卵巢、胸腺等脏器重量（P0.05,P0.01）,显著提高E2、P含量（P0.05, P0.01）,增加子宫内膜平均厚度(P0.01），改善卵巢形态。结论 海参花水提物可增加子宫内膜厚度，对卵巢早衰发挥防治作用，对雌性小鼠生殖系统有一定的保护作用。 关键词:海参花;环磷酰胺;卵巢早衰;生殖系统 doi: 中图分类号:R965 文献标志码:A 文章编号： Effects of Water Extracts of Sea Cucumber Ovum on Reproductive System of Premature Ovarian Failure Mice* ABSTRACT:OBJECTIVE To investigate the protective effects of water extracts of sea cucumber ovum on deficiency of the genital system injured by cyclophosphamide. METHODS female kunming mice were selected and randomly divided into 6 groups with 10 animals in each group, except the control group injected with normal saline, others were injected with cyclophosphamide(CP, 28mg/kg) daily for successive 5 days. Lead to reproductive system disorders, premature ovarian failure in mice. All mice were given different drugs. Detected the level of estradiol (E2) and progesterone (P) by radioimmunoassay, all mice were compared the weight of organs, such as uterus, ovarian, spleen, and thymus, observed for the form of uterus and ovarian. RESULTS Sea cucumber ovum could increase the weight of organs, such as uterus, ovarian, and thymus gland of mice obviously（P0.05, P0.01）, significantly improved the level of E2 and P（P0.05, P0.01）,increased the thickness of endometrial (P0.01）, improved the form of ovarian obviously. CONCLUSION Sea cucumber ovum could increased the thickness of endometrial, prevent the premature ovarian failure(POF), promote and protect the reproductive system of female mice. KEY WORDS:sea cucumber ovum；cyclophosphamide；premature ovarian failure；reproductive system 海参（sea cucumbers)属于棘皮动物门海参纲，不但营养丰富，而且富含多种有益于人体健康的生理活性物质，素有“海中人参”之称[1]。现代研究表明，海参中含有海参多糖、海参皂苷、脂肪酸和胶原多肽等多种生物活性物质，具有抗凝血、抗肿瘤、免疫调节、延缓衰老、降血脂等多种生物学活性[2-3]。海参卵