竹叶黄酮和决明子复配维生素C.doc

竹叶黄酮和决明子复配与维生素C、E协同调节高脂血症大鼠脂代谢及抗氧化应激的研究 Regulation of Lipid Metabolism and Oxidative Stress by Bamboo Leaf Flavonoid and Cassia Seed Compound Associated with Ascorbyl Palmitate and Vitamin E Succinate in Hyperlipidemia Rats 投稿时间： 2014-03-24?? DOI： 中文关键词:??竹叶黄酮组合物??脂质代谢??抗氧化应激??协同增效 英文关键词:bamboo leaf flavonoid compound??lipid metabolism??oxidative stress??synergistic effect 基金项目:国家重点基础研究发展计划（973计划）（2012CB720806）；宁波市自然基金（2014A610201）；宁波市农业科研攻关项目（2014C10057）；宁波市重点学科项目（XKL11D2109）； 作者 单位 刘连亮? 宁波大学海洋学院，浙江宁波 315211? 吴聪俊? 浙江大学生工食品学院，浙江杭州 310058? 中文摘要: ??????本文研究了[竹叶黄酮（BLF）+决明子粉（CSF）=16:9]与维生素C棕榈酸酯（AP）、维生素Ｅ琥珀酸酯（α-TOS）的体外抗氧化活性，并考察了不同配比组方调节高血脂大鼠脂质代谢及其抗氧化应激的能力。[BLF+CSF]与AP组合（5:1）的体外抗氧化协同增效作用显著强于[BLF+CSF]与α-TOS组合（5:1）；[BLF+CSF]与AP、α-TOS组合（5:1:1）协同增效效果最为显著，表现出最强的体外抗氧化能力。构建高脂血大鼠模型，并评价不同组合物降脂和抗氧化应激能力，结果表明各组方均能够调节高脂血症大鼠的脂质代谢，提高其抗氧化应激能力。其中，[BLF+CSF]与AP和α-TOS（5:1:1）协同增效调节脂质代谢和抗氧化应激效果最为显著，可协同增效显著降低动脉粥样硬化指数（p0.01），调控血脂：降低TC（p0.01）、TG（p0.01）、LDL-c（p0.01），显著升高HDL-c（p0.01）。在体内抗氧化应激能力上：显著提高血清T-AOC（p0.01），显著增加SOD活性（p0.01）、GSH-Px活性（p0.01），降低MDA（p0.01）。 英文摘要: ??????This study was to investigate the in vitro oxidative stress activity of bamboo leaf flavonoid (BLF) and Cassia seed powder (CSF) compound (16:9, m/m), in combination with ascorbyl palmitate (AP) and Vitamin E succinate (α-TOS), as well as their ability to regulate lipid metabolism and anti-oxidative stress at different ratios in hyperlipidemic rats. Combination of [BLF +CSF] and AP with ratio of 5:1) had synergistic anti-oxidative activity, which was significantly higher than that of [BLF+CSF] and α-TOS with ratio of5:1. The combination of [BLF+CSF], AP, and α-TOS with ratio of 5:1:1 exhibited the most significant synergistic effect. A hyperlipidemia rat model was established, and the lipid-lowering and anti-oxidant abilities of different combinations were evaluated. The results showed that all combinations could effectively regulate lipid metabolism and reduce oxidative stress in hyperlipidemic rats. Th