果蠅RNAi的实验中双链短RNA的合成.docVIP

果蝇RNAi的实验中双链短RNA的合成（dsRNA)方法 本文来自于哈佛大学医学院果蝇RNAi筛选中心的经典HYPERLINK /protocol/实验方法，专门用于果蝇RNAi实验方法。感谢哈佛大学医学院果蝇RNAi筛选中心的支持！ Primer Designed dsRNA Template Selection PCR In vitro RNA Transcription dsRNA Purification, Quantification, Storage dsRNA From Clones PCR In vitro RNA Transcription Purification, Quantification, Storage Additional References We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Designed dsRNA). It is also possible to produce dsRNA using PCR generated DNA templates containing either the