胃癌組织基质金属蛋白酶7基因表达与门静脉血道转移的关系.doc

KKME---专业医学搜索引擎/ 胃癌组织基质金属蛋白酶-7基因表达与门静脉血道转移的关系 　　作者：张长乐,项和平 　　【摘要】 目的 研究胃癌组织基质金属蛋白酶-7(MMP-7)基因表达与胃癌门静脉血道转移及胃癌病理特征之间的关系,探讨其临床意义。方法 采用逆转录聚合酶链反应(reverse transcriptase-polymerase chain reaction,RT-PCR)方法检测30例胃癌患者癌组织和正常黏膜MMP-7mRNA，采用RT-PCR方法检测30例胃癌患者和10例腹腔良性疾病患者门静脉血中细胞角蛋白20的mRNA(CK20mRNA)。结果 胃癌组织MMP-7mRNA表达率为60%(18/30)，明显高于远端正常组织的(4/11)11%(P0.001);胃癌组有MMP-7基因表达的门静脉血CK20mRNA检出率达83.3%(15/18)，显著高于无MMP-7基因表达的(1/12)8.3%(P0.001);MMP-7基因表达与组织分化、TNM分期和肿瘤浸润深度相关(P0.05);对照组门静脉血中CK20mRNA均为阴性。结论 MMP-7基因表达与胃癌血行转移有关，参与了胃癌的浸润、转移过程，有助于判断预后，MMP-7基因表达阳性的胃癌患者应尽早行全身化疗。 　　【关键词】 胃肿瘤;肿瘤细胞;聚合酶链反应;细胞角蛋白20;基质金属蛋白酶-7;转移 　　Expression of MMP-7 gene in gastric cancer tissues and its correlation with metastasis in portal vein blood 　　ZHANG Chang-le,XIANG He-ping. 　　Department of Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230000, China 　　【Abstract】 Objective To study the expression of matrix metalloproteinase-7 (MMP-7) mRNA in gastric cancer and the relationship with clinicopathologic factors and metastasis in portal venous blood,explore its clinical significance.Methods Reverse transcriptase-polymerse chain reaction(RT-PCR)was used to detect the MMP-7 mRNA in tumor tissues and in the surrounding normal tissues of 30 patients with gastric cancer,RT-PCR was used to detect the CK20 mRNA in portal venous blood of 30 patients with gastric cancer and 10 patients with benign disease who underwent laparotomy served as controls.Results The positive rate of MMP-7 mRNA in tumor tissues 60%(18/30)were significantly higher than those the surrounding normal tissues 11%(4/11),(P0.001). The positive rate of CK20 mRNA of portal venous blood in patients with MMP-7 mRNA expression(83.3%,15/18)were significantly higher than those without MMP-7 expression (8.3%,1/12),(P0.001). The overexpression of MMP-7 mRNA was also related to different gastric cancer cell line,the TNM classification and the stage of the cancer (P0.05).None of 10 patients with benign disease was posit