GrowthCultureofBacteria.pptVIP

Chapter 6 Growth Culture of Bacteria Binary Division 1 to 2 to 4 to 8 to ? Phases of Growth Lag Adapt to nutrients Log Active growth Stationary Death = Growth rate Death Nutrients consumed pH too low (why?) Optimize curves in production Measuring Growth Direct Counts Petroff-Hauser Chamber Pro’s vs. Con’s Serial Dilution 10-fold serial dilutions Pro’s vs. Con’s MPN (Most Probable Number) Put 10, 1, and 0.1 ml into 10-mls broth Repeat 5 times per volume Statistical accurate sampling Public Health Standards are written for MPN Physical Factors: -philes pH Acido-, Neutro-, Alkaliphiles Canned f