ProkaryoticExpression课程.pptVIP

pET Expression System Protein Purification by Ni-NTA Postgraduate : Cai Fuqiang Professor: Wang Yanlin Prokaryotic Expression Prokaryotic Expression Prokaryotic Organism Lac Operon Model Founders: 1961 Jacob and Monod et al. Frontier of Gene Expressional Regulation Lac Operon Model Lactose Metabolism Lactose Operon Lac I: Repressor Protein Lac P: Promoter Lac O: Operator Lac Z: Galactosidase Lac Y: Galactoside Permease Lac A: Galactoside Acetylase pET E.coli Expression System Novagen’s pET System The most powerful system T7promoter-driven expression system Host strains