香菇菌丝体不同氮源的利用研究.docVIP

香菇对不同氮源的利用研究 周振荣，生命科学学院 摘 要采用液体深层培养方法，分析了六种氮源（豆粉，豆渣粉，硝酸钾，硝酸铵，硫酸铵和尿素）对香菇菌丝生长的影响。实验结果表明：有机氮源比无机氮源更利于香菇菌丝的吸收生长。在实验所选用的六氮源中，豆粉、豆渣粉的效果都比较好，菌球密度大且大小均匀，72小时培养后，每100mL培养液中菌丝干重分别为0.583mg和0.587mg而且以豆渣粉为氮源最为经济且最易获得，菌丝干重最高；在无机氮源中，以硝酸钾为氮源的培养液中香菇的菌丝球大且多，菌丝干重为0.487mg。然而以硝酸铵，硫酸铵和尿素为氮源的培养基中，香菇的菌丝几乎没有生长，和接种时差不多，菌丝干重分别为0.027mg、0.023mg和0.024mg，菌丝球呈休眠状态。初步研究表明通常废弃的豆渣粉可以用来作为香菇菌种液体深层培养的氮源，从而为发展食用菌产业开辟了新的思路。 关键词：香菇；氮源；深层培养；菌丝体 The Study of the Utilization of Lentinus edodesycelia to Different Nitrogen Sources ZHOU Zhen-rong, College of Life Sciences Abstract: Submerged culture was used to study the effects of six nitrogen sources (soya flour, bean dregs powder, KNO3, NH4NO3, (NH4)2SO4 and urea) on the mycelial growth of Lentinus edodes. The results showed that: the mycelia of Lentinus edodes was more adaptable to organic nitrogen sources than inorganic nitrogen sources. Among the organic nitrogen sources, both soya flour and bean dregs powder had good effects. In every 100mL liquid culture media, the weight of the dry mycelia in soya flour treatment was 0.583mg, in bean dregs powder was 0.587g. The densities of mycelia were large and the sizes of mycelia balls were homogeneous. However, when bean dregs powder was used as nitrogen source, the weight of the dry mycelia was the highest one. Furthermore, bean dregs powder was the most economical one and it was easy to obtain. Among the inorganic nitrogen sources, the mycelia balls of Lentinus edodes in KNO3 treatment were big and the mycelia balls number was large, the weight of the mycelia was 0.487g. However, the weight of the dry mycelia in NH4NO3 treatment was 0.027g, in (NH4)2SO4 treatment was 0.023g, and in the treatment of urea was 0.024g, from which we could see that the mycelia could hardly grow and the mycelia balls were dormant. The primary study indicated that the waste bean dregs powder could be used as the nitrogen source for Lentinus edodes, which opened a new way for the development of the edible mushroom. Keywords: