DTPA xtraction from micronutrients.docVIP

DTPA Extraction for Fe, Zn, Cu, Mn and DTPA-Sorbital Extraction for Fe, Zn, Cu, Mn and B Tony Provin and Hailin Zhang Application and Principle First developed and published by Lindsay and Norvell (1978) for extracting micronutrients in neutral and calcareous soils, the DTPA extraction method has gained significant adoption by agricultural laboratories. While originally conceived to address the over-estimation of micronutrients by acid extracts in calcareous soils, the equilibrium/chelation mechanism coupled by the use ICP analysis has proven useful in nearly all pH ranges. Plant available boron in the western United States has historically been analyzed via refluxing boiling water, a procedure requiring significant technician involvement and safety hazards. Vaughan and Howe (1994) reported on the potential for using boron chelates instead of refluxing water. As a result, Miller et al. (2000) documented the inclusion of the sugar sorbital in the DTPA extractant for plant available Fe, Zn, Cu, Mn and B simultaneous extraction and determination. Equipment and Apparatus 1. Digital balance (0.0000 gram resolution) 2. Digital balance (0.00 gram resolution) 3. 100 ml graduated cylinder 4. 500 ml graduated cylinder 5. 20 L poly carboy 6. 1.5-2” Teflon stir bar 7. Inline (180 rpm-2-2.5” throw or orbital shaker (180 rpm with 1” throw) either shaker must be fitted with appropriate sample vessel holders 8. 250 ml Erlenmeyer flask or similar extracting vessel 9. Filter rack 10. Dispenser capable of rapid dispensing of 10-20 ml of extractant to 0.1ml resolution 11. Calibrated pH meter Reagents 1. Deionized water 2. Diethylenetriaminepentaacetic acid (DTPA) 3. Triethanolamine (TEA) 4. Calcium chloride (CaCl2) 5. HCl (12M) 6. ICP or ICP/MS grade 1000 ppm Zn, Fe, Cu, Mn and B standards dissolved in HCl or nitric acid 7. In addition to above, for DTPA-Sorbital method include: Sorbitol (D-So