基于UPLC-Q-TOF-MS技术分析石吊兰全草中的化学成分.docVIP

基于UPLC-Q-TOF-MS技术分析石吊兰全草中的化学成分 胡峻1，齐梦蝶 1，张权1，秦振娴1，康利平2，南铁贵2，杨健2，袁媛2，詹志来2*，刘勇1* （1.北京中医药大学，北京 100102；2. 中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地， 北京 100700） [摘要] 目的：建立石吊兰全草化学成分的一种超高效液相色谱-四极杆飞行时间串联质谱联用技术分析方法。方法： 80％甲醇-水溶液超声提取石吊兰，经Waters ACQUITY UPLC-BEH-C18S色谱柱（100 mm×2.1 mm，1.7 μm），以0.1％甲酸水-乙腈为流动相梯度洗脱，检测波长210～400 nm，ESI负离子模式检测，根据精确分子量及碎片信息，结合数据库匹配进行结构鉴定。结果：从石吊兰全草中共鉴定出48个成分，其中42个苯乙醇苷类化合物，3个黄酮类化合物，3个其他类型化合物，其中有14个化合物为苦苣苔科中首次发现。结论：本研究首次对石吊兰化学成分进行整体研究，为该植物的深入研究奠定基础。 [关键词] 液质联用；石吊兰；化学成分 Study on chemical constituents in the whole herb of Lysinotus pauciflorus by UPLC-Q-TOF-MS HU Jun1, QI Mengdie1,ZHANG Quan1, QIN Zhengxian, KANG Liping2, NAN Tiegui2, YANG Jian2, YUAN Yuan2, ZHAN Zhilai2*, LIU Yong1*. (1. Beijing University of Chinese Medicine, Beijing 100102, China； 2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China) [Abstract] Objective:To analyze the chemical components in the whole herb of Lysinotus. pauciflorus Maxim by Ultra high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF- MS). Methods: Crude drug were extracted with 80% methanol in water. A Waters ACQUITY UPLC-BEH- C18S column（2.1 mm×100 mm, 1.7 μm）was used with a gradient elution of acetonitrile–water containing 0.1％ formic acid. The mass spectrometry equipped with ionization source was used and the data was collected under negative ion mode. And the components identification was applied by the precise molecular weight and fragmentation information, combined with a database match. Results: Fouty-eight compounds were identified with 42 phenylethanoid glycosides, 3 flavonoids and 3 other compounds, among them, 14 compounds were firstly identified in Gensneriaceae plant. Conclusion:We have quite completely identified the components in the whole herb of L.. pauciflorus for the first time, which lay the foundation for the further study and utilization of the