C_止痛、麻醉、術前與術後照顧.docVIP

IACUC參考資料C 止痛、麻醉、術前與術後照顧 目的 These guidelines apply to all surgical procedures performed on rodents in which the animals are expected to recover from anesthesia. All staff working with animals is trained to recognize animal pain or distress, and know appropriate action to relieve pain or who to advise. 此規範的目的在確保術後的嚙齒類實驗動物得以順利恢復。同時讓動物使用者可於疼痛發生時採取適當的措施。 名詞解釋 Survival Surgery procedures can be divided into: 存活性手術大致可分為兩類： Minor Survival Surgery that is defined as any surgical intervention that does not penetrate a body cavity nor results in a permanent handicap in an animal that is expected to recover. Minor procedures are often performed under less stringent conditions than major procedures but still require aseptic technique and instruments and appropriate anesthesia. 次要存活性手術，指不侵入體腔或對身體機能、且不會造成永久性之損傷的手術。相較於主要存活性手術，次要存活性手術的操作條件較不嚴苛，但仍須符合無菌操作標準，並給予適當的麻醉。 Major Survival Surgery includes invasion of the cranial, abdominal or thoracic cavities. Any procedure that might leave the rodent with a permanent handicap, whether physical or physiological, would also be considered major surgery. The use of aseptic technique is mandatory in these surgeries to minimize the possibility of post-surgical infection. 主要存活性手術，指的是侵入頭顱、腹腔或胸腔的手術或對身體機能會造成永久性之損傷的手術。執行主要存活性手術，除了必須無菌操作，同時也需盡可能降低術後感染的機會。 PROCEDURES Minor Surgery On Rodent 嚙齒類實驗動物次要存活性手術 Instruments are sterilized prior to surgery. 所有器械應於手術前完成消毒。 The surgical area should be disinfected with a 70% alcohol or quaternary ammonium compounds prior to surgery. 手術操作區需以70%酒精或四級胺類消毒劑進行消毒。 Animal should be free of clinical signs of disease. 動物必須沒有任何臨床症狀。 Prior to skin penetration, animal fur is shaved and skin cleaned and disinfected with 70% alcohol or surgical iodine. 手術區域的毛髮應於術前剃除乾淨，同時應清潔皮膚，並以70%酒精或手術專用碘液進行消毒。 The surgeon should wear sterile gloves and surgical masks. If performing batch surgeries, gloves should be changed. 手術操作人員應穿戴無菌手套及手術口罩。若連續進行多項手術，應於不同手術間更換手套。 Major Survival Surgery On Rodent 嚙齒類實驗動物主要存活性手術 Animal Prepar