T细胞亚群检测题库.pptVIP

* * 流式细胞仪通过前向散射与侧向散射、荧光信号这两种属性将细胞进行分群、分析的。 * Dichroic,二色的 fliters Bandpass filters * Their fluorescence emission wavelengths are: FITC = 530 nm, PE = 575 nm, PE–CY5 = 670 nm PE–TR = 613 nm, phycoerythrin–cyanine 5 tandem complex:PE-CY5 fluorescein isothiocyanate (FITC), phycoerythrin (PE), * Their fluorescence emission wavelengths are: FITC = 530 nm, PE = 575 nm, PE–CY5 = 670 nm PE–TR = 613 nm, phycoerythrin–cyanine(花青)5 tandem complex:PE-CY5 fluorescein isothiocyanate (FITC),异硫氰酸荧光素 phycoerythrin (PE),藻红蛋白 Detection of T cell subtypes by Flow Cytometry * BD公司流式细胞仪 FACSCalibur LSRII FACSCan