肺癌组织中低氧诱导因子1α和糖转运蛋白1的表达及其临床意义.doc

摘要】 目的 探讨肺癌组织中低氧诱导因子1α（HIF1α）和糖转运蛋白1（GLUT1）的表达及其临床意义。方法 采用免疫组织化学方法检测42例（男28例，女14例，平均年龄54.7岁，其中小细胞癌2例，鳞癌21例，腺癌16例，细支气管肺泡癌3例）肺癌组织中HIF-1α、GLUT1的表达。结果 42例肺癌组织中HIF-1α阳性表达35例（83.3%）；GLUT1阳性表达36例（85.7%）。肺癌组织T3、T4组与T1、T2组相比HIF-1α、GLUT1高表达率均明显增加，差异有显著性（分别P＜0.025，P＜0.05）。肺癌组织有淋巴结转移组与无淋巴结转移组相比，HIF-1α、GLUT1高表达率均明显增加，差异有显著性（分别P＜0.025，P＜0.05）。肺癌组织中HIF-1α表达与GLUT1表达有显著正相关性（P＜0.01）。结论 肺癌组织中HIF1α与GLUT1蛋白高表达，两者显著正相关，HIF1α可能对GLUT1的表达起上调作用。HIF-1α与GLUT1高表达与肺癌的浸润程度和淋巴结转移有关。 【关键词】 肺癌；低氧诱导因子1α；糖转运蛋白1；免疫组化 　　The expression and clinical significance of hypoxia induced factor 1α and glucose transporter 1 in lung carcinoma 　　SONG Guang-Yao, ZHANG Jie-Ying, HE Chun-Nian, et al. 　　Hebei Provincial People’s Hospital, Shijiazhuang 050051, Hebei, China 　　【Abstract】 Objective To investigate the expression and clinical significance of hypoxia induced factor 1α (HIF-1α) and glucose transporter 1 (GLUT1) in lung carcinoma. Methods The expression of HIF-1α and GLUT1 were tested by immunohistochemical method in 42 cases of lung cancer specimens (28 males and 14 females, with an average age of 54.7. Pathologically, 2 cases small cell carcinoma, 21 cases squamous carcinoma, 16 cases adenocarcinoma, and 3 cases alveolar carcinoma). Results In 42 cases of lung carcinoma specimens, 35 cases showed positive staining of HIF-1α protein (83.3%); 36 cases showed positive staining of GLUT1 protein (85.7%). The expression rate of HIF-1a and GLUT1 in lung carcinoma tissues of T3 and T4 groups was markedly higher than that in T3 and T4 group (P＜0.025, P＜0.05 respectively). The expression of HIF-1a and GLUT1 with lymphatic metastasis in lung carcinoma was significantly higher than those without lymphatic metastasis (P＜0.025, P＜0.05 respectively). Significant positive correlation between the expression of HIF-1α and GLUT1 was seen in lung carcinoma (P＜0.01).Conclusions High expression of HIF1α and GLUT1 at protein level could be seen in human lung cancer, and there is significant positive correlati