脂联素基因SNPs(tougao).docVIP

脂联素基因SNPs＋45 TG和SNPs＋276GT与老年非糖尿病冠心病的相关性 摘要 目的：探讨老年人脂联素基因SNPs＋45 TG和SNPs＋276GT与老年非糖尿病冠心病的相关性。对象与方法：选择2005年11月至2009年12月入住我院心内科病房，行冠状动脉造影、年龄大于等于65岁的非糖尿病患者688名，根据冠状动脉造影结果分为冠心病组396例，对照组292例。采用PCR/LDR法检测多态性位点。结果：SNPs＋45 TG基因表型为突变型GG者发生冠心病的危险性较具有TT型者显著增加（OR值为2.65，p0.01）；SNPs＋276GT基因表型为GG型者发生冠心病的危险性较具有TT型者显著增加（OR值为2.36，p=0.001，0.01）；杂合子GT型者发生冠心病的危险性也较具有TT型者显著增加（OR值为1.66，p=0.049，0.05）。logistic回归分析显示，SNPs+45TG基因表型为GG型，SNPs +276 GT基因表型为GG和GT型是冠心病发病独立的危险因素。SNPs +45TG和SNPs＋276GT位点存在连锁不平衡，脂联素基因SNPs＋45 TG基因表型为突变型GG型者SNPs+276 GT基因表型均为GG型，而SNPs+276 GT基因表型为突变型TT型者SNPs＋45 TG基因表型均为TT型，即GGGG基因表型和TTTT基因表型。且SNPs +45TG和SNPs＋276GT位点基因表型为GGGG型者发生冠心病的危险性显著高于TTTT型者，OR值为4.77（p=0.000，p0.01）。结论：在老年非糖尿病者中，脂联素基因SNPs＋45 TG基因表型为GG型者，SNPs+276 GT基因表型为GG或GT型者可能是冠心病的易感人群。 关键词： 脂联素 单核苷酸基因多态性 冠心病 老年 The Relationship Between Two Single Nucleotide Polymorphism (+45 TG and +276 GT) of Adiponectin Gene and Coronary Artery Diseases in Elderly Patients without Diabetes Abstract: Objective: To investigate the association between two single nucleotide polymorphism (SNPs+45 TG and SNPs +276 GT) of adiponectin gene and coronary artery diseases in elderly patients without Diabetes. Subjects and Methods: Coronary angiograms in 688 subjects aged 65 years or older without Diabetes were performed during Nov 2005 to Dec 2009. Genotyping was done for two SNPs by polymerase chain reaction/ligase detection reaction (PCR/ LDR) assay. Results: At +45 position, the risk rate of CAD in GG genotype is significantly distinct with that in TT genotype. OR (odd rate) of GG genotype is 2.65(p0.01). At +276 position, the risk rate of CAD in GG or GT genotype is significantly distinct with that in TT genotype. OR (odd rate) of GG and GT genotype is 2.36 (p0.01) and 1.66 (p0.05). Logistic regression analysis shows that GG genotype at SNPs +45 TG, GG genotype and GT genotype at SNPs +276 are independent risk factors of CAD. Furthermore, adiponectin gene SNPs+45 TG and SNPs +276 GT were displayed strong linkage disequilibrium. H