P物质对体外培养成骨细胞增殖和活性的影响.docVIP

P物质对体外培养成骨细胞增殖和活性的影响 【关键词】 骨细胞 Effect of substance P on proliferation and activity of osteoblasts in vitro 【Abstract】 AIM: To observe the effect of substance P on the proliferation and activity of osteoblasts cultured in vitro. METHODS: The calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observ