Restin基因启动子的克隆及其在HeLa细胞中的转录活性.docVIP

Restin基因启动子的克隆及其在HeLa细胞中的转录活性 作者：王瑞华，傅海燕，杨国栋，路凡，赵忠良 【关键词】 全反式维甲酸 Molecular cloning of restin gene promoter and its transcriptional activities in HeLa cells 【Abstract】 AIM: To clone the promoter and construct its luciferase report vector of our newly found gene restin and to analyze its response to the stimulation with alltransretinoic acid . METHODS: Approximate 2000 bp up from the ATG of gene restin was bioinformatics analyzed with computer. The fragment was generated by polymerase chain reaction and cloned the upstream of lucife