RNA干扰对流感病毒NP和PA基因表达及病毒增殖的抑制.docVIP

RNA干扰对流感病毒NP和PA基因表达及病毒增殖的抑制 作者：李瑶琛，赵志刚，李康生 【关键词】 正粘病毒科 RNA interference mediated inhibition of influenza virus NP and PA gene expressions and multiplication in MDCK cells 【Abstract】 AIM: To apply the small interfering RNAs targeting NP or/and PA gene of influenza virus to inhibit the expression of NP or/and PA and multiplication of influenza virus in MadinDarby canine kidney cells. METHODS: The recombinant plasmids, pEGFP/NP, pGenesil/PA and pEGFP/NP+ PA were constructed and transfected into MDCK cells. The transfected MDCK ce