EBNA2基因真核表达载体的构建及表达产物的功能.docVIP

EBNA2基因真核表达载体的构建及表达产物的功能 【关键词】 EBNA2 Construction of eukaryotic expression vector of EBNA2 and functional detection of its product 【Abstract】 AIM: To construct the eukaryotic expression vector of EBNA2 and to express it in eukaryotic cells and detect the function of its product. METHODS: EBNA2 gene was obtained by PCR and DNA recombination from the plasmid pSG5EBNA2. PCR product of EBNA2b was then inserted into plasmid pMD18T and sequenced. The full length EBNA2 gene was cut and inserted into eukaryotic expression plasmid pCMVHA. U