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异丙酚下调脂多糖诱发的大鼠肺泡Ⅱ型上皮细胞TLR4的表达 马铃 洪涛 陈卫民 （中国医科大学附属盛京医院麻醉科，沈阳，110004） [摘要] 目的 探讨不同浓度异丙酚对脂多糖诱发的大鼠肺泡II型上皮细胞（ATⅡ）免疫作用机理。 方法 原代培养大鼠ATⅡ细胞，随机分为五组：C组：对照组，不添加任何药物，培养3小时；LPS组：1μg. ml-1 LPS培养3小时；P1 组：1μ.ml-1 LPS 和25μM异丙酚培养3小时；P2 组：1μg.ml-1 LPS 和50μM异丙酚培养3小时；P 3 组：1μg.ml-1 LPS 和100μM异丙酚培养3小时。测定ATⅡ细胞TLR4 mRNA和蛋白含量，培养基上清中TNF-α含量。结果 1μg.ml-1 LPS增加ATⅡ细胞TLR4 mRNA和蛋白的表达，增加TNF-α含量（P0.05）。50μM和100μM异丙酚能下调LPS 引起的ATⅡ细胞TLR4 mRNA和蛋白表达的增加，抑制LPS引起TNF-α含量的增高（P0.05）。结论 异丙酚可能通过剂量依赖性的抑制 LPS引起的ATII细胞的TLR4 mRNA和蛋白表达，减少TNF-α含量从而减少肺组织炎症反应。 [关键词] 肺泡II型上皮细胞；二异丙酚；Toll样受体；肿瘤坏死因子-α 第一作者单位：马铃，博士在读。工作单位：中国医科大学附属盛京医院。 通信作者：陈卫民，沈阳市和平区三好街36号，电话：024Email:chenwm@sj-hospital.org 正文字数： 2946字 图数：6 Propofol downregulates LPS-induced TLR4 expression in rat alveolar type II epithelial cells Ma Ling, et al. Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang, 110004, China [Abstract] Objective To investigate whether LPS induced inflammation in ATII is through TLR4 and the effect of different dosage of propofol on the inflammation. Methods The primarily cultured ATII cells were randomly assigned to one of the following five groups: Group C: AT II cells in untreated group (control) was cultured for 3 h; Group LPS: treated with 1μg. ml-1 LPS for 3 h; Group P1: treated with 1μ.ml-1 LPS and 25μM propofol for 3 h; Group P2: treated with 1μg.ml-1 LPS and 50μM propofol for 3 h; Group P3: treated with 1μg.ml-1 LPS and 100μM propofol for 3 h. Real time PCR was used to detect TLR4 mRNA expression while western blot were used to measure the relative TLR4 protein expression. TNF-α levels in supernatant were measured using ELISA. Results 1μg. ml -1 LPS significantly increased TLR4 mRNA and protein expression, as well as TNF-α levels in AT II cells (P0.05). Propofol at 50 and 100μM significantly inhibited the increase in TLR4 mRNA and protein expression, and TNF-α levels induced by LPS (P0.05). Conclusion Propofol can dose-dependently inhibit inflammatory