表面活性剂TW-80与城市景观河道污染底泥中多环芳烃微生物降解之间.docVIP

表面活性剂TW-80与城市景观河道污染底泥中多环芳烃微生物降解之间的作用分析 王晓欧 2012214073 苏溦娜 2012214112 龚克娜 2012214063 郑博一 2012214131 尚轩 2012214110 原国栋 2012214126 摘要 本文通过室内模拟实验对表面活性剂（TW-80）与城市景观河道污染底泥中多环芳烃微生物降解之间的作用进行了分析。研究发现表面活性剂（TW-80）能提高多环芳烃的生物可利用性，从而改善污染底泥的修复效果。但表面活性剂浓度过高时短时间内会抑制微生物的活性，经历抑制期后，微生物活性会迅速得以恢复，甚至增强，从而导致多环芳烃的总降解率显著提高，在实际应用中，TW-80的浓度控制在400mg·kg-1以下比较理想。PAHs的生物降解受其分子量、分子结构、表面活性剂浓度的影响。经对TW-80与污染底泥中PAHs微生物降解之间的动力学分析发现：天然状态的底泥中PAHs的微生物降解为零级反应；在只投加外源菌种、不投加TW-80的情况下， PAHs的微生物降解为二级反应；在投加外源菌种和TW-80的情况下，PAHs的微生物降解绝大多数为一级反应，TW-80=1000mg/L时蒽、芘的微生物降解为二级反应，TW-80=10000mg/L时荧蒽、芘、总PAHs的微生物降解为零级反应，当TW-80浓度过高时对荧蒽、芘的微生物降解的抑制作用更大。 关键词：表面活性剂 TW-80 多环芳烃(PAHs) 微生物降解 动力学分析 Abstract In this article, the function between surfactant (TW-80) and microbial degradation of polycyclic aromatic hydrocarbons in urban landscape river’s polluted sediments were analyzed by indoor simulation. Study found that the surfactant (TW-80) can improve the bioavailability of the polycyclic aromatic hydrocarbons (PAHs), therefore it can improve the restoration effect of the polluted sediments. But if the concentration of the surface active agent went too high, the microbial activity would be inhibited in a short time. After the period of inhibition, the microbial activity would be quickly restored and even enhanced, resulting a significant increase of PAHs total degradation rate. It would be relatively ideal to control concentration of TW -80 under 400mg ? kg-1 in practical applications. The biodegradation of PAHs was influenced by their molecular weight, molecular structure, the surfactant concentration. Analysis of the dynamics between the TW-80 with contaminated sediments’ PAHs microbial degradation found: the microbial degradation of PAHs is the zero-order reaction under natural state of the sediments’ PAHs; it would be second-order reaction when dosing only the exogenous strains and no TW-80; and the vast majority of first-order reaction with both exogenous strains and TW-80; when TW-80 = 1000mg