流式细胞仪-上课-2014说课.ppt

* * * 17 * * * * * Doublets * * * * In A, the amount of fluorescence emission detected by the PE detector and the amount of PE fluorescence detected by the FITC detector is shown. After compensation, B, the particles show only their appropriate fluorescence emission. * * Combining signals from the green-fluorescent JC-1 monomer (absorption/emission maxima ~510/527 nm) and the red-fluorescent J-aggregate ( ) (absorption/emission maxima ~585/590 nm), For flow cytometry, JC-1 can be excited at 488 nm and detected using the green channel for the monomer and the red channel for the J-aggregate