MB-4-replication例析.ppt

2) Rolling Circle Replication Circular DNAs can replicate by a rolling circle mechanism One strand of a dsDNA is nicked and the 3’-end is extended This uses the intact DNA strand as a template The 5’-end is displaced Phage ?X174 replication cycles so that when one round is complete a full-length, single-stranded circle of DNA is released Phage l, displaced strand serves as the template for discontinuous, lagging strand synthesis Phage l Rolling Circle Model As the circle rolls right Leading strand elongates continuously Lagging strand elongates discontinuously Uses unrolled leading strand as a