guan常用技术.pptVIP

（三）气体 细胞的生长代谢自然离不开气体，容器空间中的O2及CO2足以保证细胞体内代谢活动的进行，但作为代谢产物的CO2在培养环境中还有另一个重要作用：即调节pH的作用 。 Medium Dissolved gases to control pH together with NaHCO3 H2O + CO2 ? H2CO3 ? H+ + HCO3- Handerson-Hasselbach eqn predicts pH: pH = 6.0966+log([HCO3- ]/[CO2 ]) 5-10% ambient CO2 dissolves in the medium ? decreasing pH addition of NaHCO3 ? increases HCO3- thus promotes H2C03 formation increasing pH Medium Dissolved gases What if 5% ? 10%? pH drops to 7.2 from 7.4 Oxygen consumption by cells: delivery is different than in vivo diffusion as opposed to hemoglobin transport limite