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Ginseng Ginsenoside Production and Morphological Characterization of Wild Ginseng (Panax ginseng Meyer) Mutant Lines Induced by γ-irradiation (60Co) of Adventitious Roots Adventitious roots were treated with different dosages of γ-ray (5, 10, 25, 50, 75, 100, and 200 Gy), in order to improve its ginsenoside content. Adventitious roots were researched to contain more ginsenoside than other part of Panax ginseng Meyer. When the intension of γ-ray over 100 Gy, the growth of adventitious roots was inhibited. The irradiated adventitious roots showed significant variation in the morphological parameters and crude saponin content at 50 to 100 Gy. Four cell lines out of 35 cell lines treated with 50 Gy and 100 Gy were selected on the basis of phenotypic morphology and crude contents relative to the wild type control. The crude saponin content of cell line 1 in flasks and bioreactor cultures increased by 1.4 and 1.8- folds compared to the control. Total contents of 7 ginsenoside types (Rg1, Re, Rb1, Rb2, Rc, Rf, and Rd) of cell line 1 increased by 1.8 and 2.3-fold compared to control. Crude saponin and ginsenoside contents in the bioreactor culture increased by about 1.4-fold compared to that the flask culture. The role of auxin is crucial for P. ginseng secondary root induction and elongation. The number of secondary roots increased considerably at the concentrations of 2 mg/L NAA and 1 mg/L IAA on MS solid medium on petridishes (Table 1). The growth rate of the cultures increased, reaching a maximum in MS liquid medium in flasks containing the concentrations of 2mg/L NAA and 0.25 mg/L IAA. (Fig. 2) In conclusion, results showed that the mutant lines induced by γ-ray irradiation (60Co) were characterized by superior morphological qualities and efficient production of second metabolites such as ginsenoside. According to the observations, the highest ginsenoside content was achieved with the mutant cell line 1. And cell line 1 can be used for additional studies.