天然产物化学英文版.doc

Effect of Oryzanol and Ferulic Acid on the Glucose Metabolism of Mice Fed with a High-Fat Diet Myoung Jin Son, Catherine W. Rico, Seok Hyun Nam, and Mi Young Kang Abstract: The effects of oryzanol and ferulic acid on the glucose metabolism of high-fat-fed mice were investigated. Male C57BL/6N mice were randomly divided into 4 groups: NC group fed with normal control diet; HF group fed with high-fat (17%) diet; HF-O group fed with high-fat diet supplemented with 0.5% oryzanol; and HF-FA group fed with high-fat diet supplemented with 0.5% ferulic acid. All animals were allowed free access to the experimental diets and water for 7 wk. At the end of the experimental period, the HF-O and HF-FA groups exhibited significantly lower blood glucose level and glucose-6-phosphatase (G6pase) and phosphoenolpyruvate carboxykinase (PEPCK) activities, and higher glycogen and insulin concentrations and glucokinase (GK) activity compared with NC and HF groups. The results of this study illustrate that both oryzanol and ferulic acid could reduce the risk of high-fat diet-induced hyperglycemia via regulation of insulin secretion and hepatic glucose-regulating enzyme activities. Keywords: diabetes, ferulic acid, high-fat-fed mice, hypoglycemic effect, oryzanol Introduction Chronic consumption of a high-fat diet has been associated with the development of obesity and type 2 diabetes mellitus (Hill and others 1992; Bray and others 2004). Scientific studies have shown that excessive intake of dietary fat results in increased body weight and poor glucose regulation (Alsaif and Duwaihy2004; Petro and others 2004; Messier and others 2007). Diabetes is characterized by hyperglycemia that results in the generation of free radicals leading to oxidative stress (West 2000). Due to changes in lifestyle patterns, particularly poor eating habit and sedentary lifestyle, the incidence of diabetes has rapidly increased in epidemic proportions. Around 171 million cases of diabetes worldwide were reported